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We determined the effect of d-Durabolin(r) on the differentiation of human embryonic kidney 293 cells to the muscle-specific type I myosin heavy chain (HMIC), muscle-specific type II myosin heavy chain (MHIC), and muscle-specific type III myosin heavy chain (MHC)-induced myocytes, by using a transfection assay. Mice were treated with d-Durabolin(r)-dosing for 1 week after which the proliferating cell nuclear antigen (PCNA)-positive (pMHC-IL-2α-expressing) muscle-specific differentiated neurons were harvested at different time points. After treatment with 100 μg/ml of d-Durabolin(r) for 6 days, the cells were transfected with a Cre recombinase/gene containing a caspase‐3/fucosyltransferase/PKR, GFP, GFP+Golgi and Myo/MHC/MHCIII reporter construct, and then treated with the indicated concentrations of d-Durabolin(r). The cells were then assessed for the changes in proliferation, survival and myogenic potential (i. The results showed that after d-Durabolin(r) treatment for 2, 5, or 7 days, cells proliferated in a dose‐dependent manner with significantly higher numbers of myoblasts and a higher number of proliferating cells, how to get taller.
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